✦ LIBER ✦

Chromosomal location of genes participating in the degradation of purines in Pseudomonas aeruginosa

✍ Scribed by Matsumoto, Hideki ;Ohta, Shin ;Kobayashi, Rieko ;Terawaki, Yoshiro

Publisher: Springer
Year: 1978
Tongue: English
Weight: 817 KB
Volume: 167
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00266910

No coin nor oath required. For personal study only.

✦ Synopsis

Genetic mapping of the genes (puu) that encode the enzymes catalysing degradation of purines in Pseudomonas aeruginosa strain PAO has been carried out. Mutants that are deficient in adenine deaminase (puuA), guanine deaminase (puuB), xanthine dehydrogenase (puuC), uricase (puuD), allantoinase (puuE), and/or allantoicase (puuF) were isolated and used for the genetic study. Conjugation by FP5 factor and generalized transduction by phage G101 gave the following map locations of these six genes on the chromosome: hisI--puuB--hisII; trpA,B--puuA--ilv202; met9011--catA1--tyu--nar9011--(puuC, puuD, puuE)--puuF. A close linkage among the puuC, puuD and puuE was demonstrated by the transduction.

📜 SIMILAR VOLUMES

Revised locations of the hisI and pru (p

Revised locations of the hisI and pru (proline utilization) genes on the Pseudomonas aeruginosa chromosome map

✍ Soldati, Leda ;Crockett, Robert ;Carrigan, Judith M. ;Leisinger, Thomas ;Hollowa 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 610 KB

The location of genes in the vicinity of the major FP2 origin on the chromosome of Pseudomonas aeruginosa PAO has been revised. The markers hisl (a transduction group of histidine biosynthetic genes) and pru (a gene cluster encoding proline utilization functions) were located in the 90 to 95/0 min c

Mapping of cysteine genes on the chromos

Mapping of cysteine genes on the chromosome of Pseudomonas aeruginosa PAO

✍ Brandt, Rowena ;Günther, Elisabeth ;Herrmann, Heidrun 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 560 KB

Three loci coding for different steps in the pathway of cysteine biosynthesis have been mapped by R68.45-mediated coconjugation analysis. The cysteine auxotrophic mutants could be subdivided into sulfite and sulfide-requiring mutants. Sulfide-requiring mutants (cysIV group) were localized at a singl

Location on the chromosome of Escherichi

Location on the chromosome of Escherichia coli of genes governing purine metabolism

✍ Jochimsen, Bjarne ;Nygaard, Per ;Vestergaard, Trygvi 📂 Article 📅 1975 🏛 Springer 🌐 English ⚖ 582 KB

Genes coding for enzymes functioning in purine salvage pathways have been located on the chromosome of Escherichia coli. The gene add encoding adenosine deaminase was located by transduction at 31 min, the gene order was established to be man-uidA-add-aroD. A deletion covering man-uidA-add was obtai

Chromosomal location of the genes for fe

Chromosomal location of the genes for ferredoxin in wheat, barley and rye

✍ C. N. Chinoy; K. M. Devos; D. Bringloe; J. C. Gray; M. D. Gale; T. A. Dyer 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 121 KB

Chromosomal location of α-amylase struct

Chromosomal location of α-amylase structural genes in rye (Secale cerealeL.)

✍ J. Salinas; C. Benito 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 312 KB

The construction in vitro of derivatives

The construction in vitro of derivatives of bacteriophage lambda carrying the amidase genes of Pseudomonas aeruginosa

✍ Drew, Robert E. ;Clarke, Patricia H. ;Brammar, William J. 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 937 KB

The amidase genes of Pseudomonas aeruginosa were inserted into a lambda replacement vector following cleavage with the restriction endonuclease HindIII. The recombinant lambdaami was detected by enhanced growth of Escherichia coli around plaques of the recombinant phage on minimal medium containing