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Chromosomal localization of ribosomal 5 S RNA genes inChironomus thummiby in situ hybridization of iodinated 5S RNA

✍ Scribed by Helmut Bäumlein; Ulrich Wobus

Publisher
Springer
Year
1976
Tongue
English
Weight
833 KB
Volume
57
Category
Article
ISSN
0009-5915

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✦ Synopsis

5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 10(7) dpm/mug with carrier-free iodine-125. After hybridization in situ of 125I-5 S RNA and autoradiography only region B3c-e (containing two "normal" and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly "heterozygous" labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorportion in spite of a continuous synthesis of 5 S RNA in salivary gland cells.

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