𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Chromophore maturation and fluorescence fluctuation spectroscopy of fluorescent proteins in a cell-free expression system

✍ Scribed by Patrick J. Macdonald; Yan Chen; Joachim D. Mueller

Book ID
113412302
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
703 KB
Volume
421
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Synthesis and maturation of green fluore
Synthesis and maturation of green fluorescent protein in a cell-free translation system
✍ Vyacheslav A. Kolb; Eugeny V. Makeyev; William W. Ward; Alexander S. Spirin πŸ“‚ Article πŸ“… 1996 πŸ› Springer Netherlands 🌐 English βš– 375 KB

A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from

Simultaneous expression and maturation o
Simultaneous expression and maturation of the iron-sulfur protein ferredoxin in a cell-free system
✍ Marcus E. Boyer; Chia-Wei Wang; James R. Swartz πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 245 KB πŸ‘ 2 views

## Abstract The model iron‐sulfur (Fe‐S) protein ferredoxin (Fd) from __Synechocystis sp__. PCC 6803 has been simultaneously produced and matured in a cell‐free production system. After 6 h of incubation at 37Β°C, Fd accumulated to >450 Β΅g/mL. Essentially all was soluble, and 85% was active. Product