Chromatography of functionalized liposomes and their components

✍ Scribed by H. Schott; B. Leitner; R.A. Schwendener; H. Hengartner

• Book ID: 104145812
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 665 KB
• Volume: 441
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)84659-8

✦ Synopsis

The antitumour drug 1 -/?-D-arabinofuranosylcytosine (ara C) was acylated by means of oleic acid anhydride, resulting in the prodrug N4-oleoyl-ara C. Together with a lipophilic biotin derivative, this lipophilic prodrug was incorporated into the bilayer membrane of unilamellar liposomes prepared by means of the detergent dialysis method. On addition of these biotinylated prodrug-liposomes to an excess of avidin, biotin residues were complexed with avidin. The unreacted avidin was removed by chromatography on an Ultrogel AcA-22 column. The prodrugliposome-avidin complex was coupled to biotinylated monoclonal antibodies through the free binding sites of the immobilized avidin. Unreacted antibodies were removed by chromatography on an Ultrogel AcA-22 column. In vitro, the liposome-antibody complexes selectively bound to cells which were recognized by the monoclonal antibodies linked to the liposomes. For this reason, a promising strategy towards a specific chemotherapy of cancer is expected.