Preparation of functional liposomes with peptide ligands and their binding to cell membranes
โ Scribed by Nobuhiro Yagi; Yoshikatsu Ogawa; Masato Kodaka; Tomoko Okada; Takenori Tomohiro; Takeo Konakahara; Hiroaki Okuno
- Book ID
- 107484793
- Publisher
- Springer-Verlag
- Year
- 2000
- Tongue
- English
- Weight
- 168 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0024-4201
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Cell penetrating peptides (CPPs) have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both __in vitro__ and __in vivo__, although the mechanisms by which the cellular uptake occurs remain unclear and controversial.
Glycosaminoglycans (GAG) were isolated from rat renal glomerular basement membranes subjected to extraction with detergents, and were quantitatively analyzed using a recently described competitive binding assay that is specific for and sensitive to microgram amounts of chondroitin and heparan sulfat
## Abstract Using phage display technology, we have isolated 12โmer peptide ligands that bind to human blood outgrowth endothelial cells (HBOEC). To avoid nonโspecific binding we apply negativeโpositive selection approach by preโincubating the library with nonโHBOEC. The selected phage clones bind