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Chromatography of dansyl-peptides from tryptic digests of electrophoretically separated proteins

✍ Scribed by Herbert Tichy

Book ID
102982148
Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
1020 KB
Volume
69
Category
Article
ISSN
0003-2697

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✦ Synopsis

With the aid of DANS-Cl it is possible to detect peptides down to 10-l' g. From electrophoresis runs, such amounts of proteins could be separated with a described electrophoresis apparatus. After tryptic digestion and dansylation the obtained peptides were run in a two dimensional chromatography system on 5 x 5 cm polyamide plates. The solvents were different concentrations of formic acid and benzene: acetic acid mixtures. The results show the reproducibility and specificity of this method for handling nanomole amounts of peptides.

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