Characterization of proteins and peptides by high-performance liquid chromatography and fluorescence monitoring of their tryptic digests

✍ Scribed by Menachem Rubinstein; Selina Chen-Kiang; Stanley Stein; Sidney Udenfriend

• Book ID: 102627953
• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 377 KB
• Volume: 95
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90193-3

✦ Synopsis

Proteins and peptides can be characterized and compared at the subnanomole level by treatment with trypsin followed by high-performance liquid chromatography on reverse-phase partition columns. A fluorescamine monitoring system automatically analyzes a portion of the column effluent while the remainder can be collected for further studies. The method has already been used for characterization of rat P-endorphin and a protein which crossreacts with antiserum prepared against prolyl hydroxylase.