A chromatographic method was employed to study the kinetics of human serum albumin (HSA) adsorbed on immobilized monoclonal antibodies. The antibodies of various specificities were covalently bound to a high-performance liquid chromatography (HPLC) silica support. For very low desorption rates, succ
Chromatographic study of the adsorption kinetics of albumin on monoclonal and polyclonal immunoadsorbents
β Scribed by C. Vidal-Madjar; A. Jaulmes; J. Renard; D. Peter; P. Lafaye
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 692 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0009-5893
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π SIMILAR VOLUMES
High performance liquid chromatography (HPLC) was used to determine the association rate constants of antigen-antibody reactions at the solid-liquid interface. The adsorption of human serum albumin (HSA) on monoclonal and polyclonal anti-HSA antibodies immobilized on a HPLC silica support was studie
of organic co-solvent generally decreases the probability The kinetic behavior of human serum albumin ( HSA ) adfor adsorption during the elution time of a protein peak sorbed on a reversed-phase support was studied. With a phos- (8). Even a partial desorption of the proteins already adphate buffer