A chromatographic method was employed to study the kinetics of human serum albumin (HSA) adsorbed on immobilized monoclonal antibodies. The antibodies of various specificities were covalently bound to a high-performance liquid chromatography (HPLC) silica support. For very low desorption rates, succ
Adsorption kinetics of human serum albumin on various types of chromatographic support
β Scribed by A. Jaulmes; C. Vidal-Madjar; A. Pantazaki
- Publisher
- Springer
- Year
- 2001
- Tongue
- English
- Weight
- 683 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0009-5893
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π SIMILAR VOLUMES
of organic co-solvent generally decreases the probability The kinetic behavior of human serum albumin ( HSA ) adfor adsorption during the elution time of a protein peak sorbed on a reversed-phase support was studied. With a phos- (8). Even a partial desorption of the proteins already adphate buffer
Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately pri
of elementary reaction steps, the adsorption of proteins to The interaction of proteins with surfaces is important in separasurfaces may embrace a large number of parallel and consection and purification procedures as well as in metabolism and its utive reaction steps, characterized by diverse bindi