Chromatographic spray for the identification of tyrosine, histidine and thir amines

✍ Scribed by Michael O'Sullivan

• Publisher: Elsevier Science
• Year: 1966
• Tongue: English
• Weight: 207 KB
• Volume: 25
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98269-x

✦ Synopsis

BENTLEY

AND WEEITE~EAD~~ who reported that the Rp values of amino acids rose (for their solvent-paper system) with an increase in the water content of the solvent. The three-fold increase in the RF values with equilibration (Table ) indicated that the "water of hydration" for the solvent-paper system employed in this study was high, ancl that omitting equilibration with Whatman No; 20 paper lowered the waterrich phase. This resulted in a different solvent composition as the moving solvent was dehydrated.

The data obtained in this study indicate a functional difference between Whatman No. 20 filter paper and the commonly employed Whatman filter papers No. I and 4. It is recommended that specific conditions be established for Whatman No. 20 paper for each solute-solvent-chamber-temperature system, It is also recommended that Whatman No. 20 paper be equilibrated for 18 to 24 h in the presence of freshly prepared solvent prior to irrigation with a critical volume of solvent.