Single-step, rapid separation of acidic
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Aurel Popa Wagner; Eugenia Psarrou; Lieselotte Popa Wagner
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Article
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1983
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Elsevier Science
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English
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A simple and rapid technique for simultaneous separation of the acidic and basic isoenzymes of horseradish peroxidase is described. Upon application of a potential gradient on the electrolyte system composed of Pharmalyte (pH 2.5-5.0) histidine (p17.64), and Ampholyte (pH 9-(1), the acidic and basic