Chromatographic separation of cyclic phosphates by means of an anion-exchange dextran gel

✍ Scribed by Genichiro Kura; Shigeru Ohashi

• Publisher: Elsevier Science
• Year: 1971
• Tongue: English
• Weight: 837 KB
• Volume: 56
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97783-5

✦ Synopsis

51S7 CHROMATOGRAPHIC SEPARATION 01; CYCLIC PHOSPHATES BY MEXNS OF AN ANION-ESCHANGE DEXTRAN GEL Cl~romatograpl~ic separation of cyclic phospl~ates on a Dowes I X4 resin or a QAE-Sephadex A-25 gel column was investigated.

Tile distribution ratios of a series of cyclic pllosphates from trimeta-to octametaphospllate were measured at various concentrations of potassium chloride, which was used as eluting agent. When the elution was carried out on a QAE-Sephades A-25 column with 0.30 Ill potassium chloride, trimeta-, tetrameta-, pentameta-and heptarnetaphospl1ate were separated from each otller and from hexameta-and octametaphosphate, but the latter two cyclic phosphates were elutcd together. These two cyclic phospllates were separated by elution wit11 0.25 M potassium cllloride. SEPARATION OF CYCLIC PI1OSPHATES ON ANION-ESCHANGE DEXTRAN GEL 115 II 'I'. A. GRANDE AND