Highly active salt-washed ribosomes from Escherichia coli are isolated by gel filtration on Sephacryl S-200 in a buffer containing 1 M ammonium chloride and putrescine, spermidine, calcium, and magnesium ions. Up to several hundred grams of cells can be processed in less than 24 h. The ribosome solu
β¦ LIBER β¦
Chromatographic Purification of Highly Active Yeast Ribosomes
β Scribed by Meskauskas, Arturas; Leshin, Jonathan A.; Dinman, Jonathan D.
- Book ID
- 118044991
- Publisher
- MyJoVE Corporation
- Year
- 2011
- Tongue
- English
- Weight
- 147 KB
- Volume
- 56
- Category
- Article
- ISSN
- 1940-087X
- DOI
- 10.3791/3214
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