𝔖 Bobbio Scriptorium
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Chromatographic Purification of Highly Active Yeast Ribosomes

✍ Scribed by Meskauskas, Arturas; Leshin, Jonathan A.; Dinman, Jonathan D.


Book ID
118044991
Publisher
MyJoVE Corporation
Year
2011
Tongue
English
Weight
147 KB
Volume
56
Category
Article
ISSN
1940-087X

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πŸ“œ SIMILAR VOLUMES


Rapid purification of highly active ribo
✍ Pierre C. Jelenc πŸ“‚ Article πŸ“… 1980 πŸ› Elsevier Science 🌐 English βš– 1000 KB

Highly active salt-washed ribosomes from Escherichia coli are isolated by gel filtration on Sephacryl S-200 in a buffer containing 1 M ammonium chloride and putrescine, spermidine, calcium, and magnesium ions. Up to several hundred grams of cells can be processed in less than 24 h. The ribosome solu

Chromatographic purification of cytoplas
✍ C. Kliffen πŸ“‚ Article πŸ“… 1970 πŸ› Elsevier Science 🌐 English βš– 504 KB

A chromatographic procedure was developed for the purification of ribosomes. Young pea plants were ground in a solvent containing polyethylene glycol, magnesium acetate, sucrose, and tris(l~ydroxymetl~yl)aminomethane-acetic acid buffer (pH 7). The slurry was poured onto a column of cellulose and san