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Affinity chromatographic purification of lentil lectin using immobilized yeast cells

โœ Scribed by Matts Ramstorp; Bo Mattiasson


Book ID
112896299
Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
442 KB
Volume
7
Category
Article
ISSN
0273-2289

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The purification of TEM-ฮฒ-lactamase from
โœ R. R. Beitle; M. M. Ataai ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 234 KB

TEM-B-lactamase was purified by immobilized metal affinity chromatography from E. coil cell extracts. It was hypothesized that this protein could be purified in one step due to the abundence of metal binding residues. A pH gradient eluted the cell extract proteins. EDTA treatment released TEM-Blacta