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Chromatographic evaluation of the equilibrium and kinetic constants for reversible complex formation in the mobile phase

โœ Scribed by A.T. Melenevskii; G.E. El'Kin; G.V. Samsonov

Book ID
108334515
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
390 KB
Volume
148
Category
Article
ISSN
1873-3778

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This paper explores the influence of mobile-phase and temperature effects on the gradient elution reversed phase chromatographic behavior of proteins. Using LiChrospher SI 500, bonded with n-butyl groups and a gradient in 1-propanol, 10 mM H3PO4, rapid separation and high mass recovery were obtained