Chromatographic differences between the cytoplasmic and the mitochondrial t-RNA of Saccharomyces cerevisiae
β Scribed by B. Accoceberry; A. Stahl
- Book ID
- 118852355
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 450 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0006-291X
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π SIMILAR VOLUMES
A conditional respiratory deficiency in yeast Saccharomyces cerevisiae is expressed as a result of a nuclear mutation in sup1 and sup2 genes (II and IV chromosomes, respectively), coding for a component of cytoplasmic ribosomes (Ter-Avanesyan et al. 1982). One such strain is studied here in detail.
The sequence organization of the yeast mit-DNA region carrying the large ribosomal RNA gene and the polar locus omega was examined. Hybridization studies using rho- deletion mutants and electron microscopy of the heteroduplexes formed between 23S rRNA and the appropriate restriction fragments, lead