Chromatin organisation and nuclear architecture in growing mouse oocytes
β Scribed by Maurizio Zuccotti; Silvia Garagna; Valeria Merico; Manuela Monti; Carlo Alberto Redi
- Book ID
- 116733624
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 474 KB
- Volume
- 234
- Category
- Article
- ISSN
- 0303-7207
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## Abstract A method is described for in vitro culture of naked growing mouse oocytes for at least four days. Tests for metabolic function indicate that the oocytes increase in volume, accumulate glucoseβ6βphosphate dehydrogenase, and maintain a steady level of ^3^Hβuridine and ^3^Hβleucine incorpo
Porcine ovarian oocytes, isolated from follicles of 5 mm in diameter (large oocytes), were fused either together or with oocytes isolated from follicles of 0.5 mm in diameter (small oocytes). In giant cells composed of two large oocytes (control) germinal vesicle breakdown (GVBD) occurred and two me