Cholangiocyte-specific rat liver proteins identified by establishment of a two-dimensional gel protein database
β Scribed by Pamela Tietz; Dr. Piet C. de Groen; N. Leigh Anderson; Christina Sims; Ricardo Esquer-Blasco; Lydie Meheus; Jos Raymackers; Martine Dauwe; Nicholas F. Larusso
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 638 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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## Abstract Rat sera were analysed by high resolution twoβdimensional electrophoresis to detect polypeptides sensitive to chemical exposure. Exposure to carbon tetrachloride, trichloroethylene or dimethylformamide increased the intensity of a polypeptide with electrophoretic characteristics (p__I__
Gel electrophoresis in combination with peptide mass fingerprinting is the method of choice for proteomic profiling of various in vitro and in vivo biological systems. In the investigation reported here we analyzed the protein composition of hepatic microsomes from untreated and phenobarbital treate