Effects of chemical exposure on rat serum proteins revealed by a modified technique of two-dimensional gel electrophoresis

✍ Scribed by Thomas Marshall; Professor Olof Vesterberg

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 412 KB
• Volume: 4
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150040510

✦ Synopsis

Abstract

Rat sera were analysed by high resolution two‐dimensional electrophoresis to detect polypeptides sensitive to chemical exposure. Exposure to carbon tetrachloride, trichloroethylene or dimethylformamide increased the intensity of a polypeptide with electrophoretic characteristics (p__I__ 4.3, isoelectric point; molecular weight, MW 45 000) similar to those of human orosomucoid. The two‐dimensional procedure was modified by using only selected gel lengths (pH range 4.5–6.5) from the first‐dimension isoelectric focusing gels. Three such gel sections were positioned side by side on the top of a second‐dimension gel slab prior to electrophoresis. Using this approach, heavily stained regions of the two‐dimensional pattern were clarified by allowing simultaneous analysis in one gel slab of the same sample at three different dilutions. With this modification other potential marker proteins for chemical exposure were detected. The advantages and application of this modified technique are discussed.