Chloramphenicol resistance in Streptomyces coelicolor A3(2): Possible involvement of a transposable element

✍ Scribed by Sermonti, Giuseppe ;Petris, Anna ;Micheli, Mariarita ;Lanfaloni, Luisa

• Book ID: 104693103
• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 433 KB
• Volume: 164
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00267604

✦ Synopsis

The transfer of a Chl element, causing resistance to chloramphenicol in Streptomyces coelicolor A3(2), was studied in NF x SCP1- superfertile crosses. When the Chl element is on the donor side (NF) its transfer to the recombinant cells was virtually total as if the element acted as a second concomitant transfer origin. When the Chl element was on the recipient side (SCP1-) it was never displaced by the immigrant chromosome even when the region facing chl+ was selected for. A fraction of the original Chl- mutants presented a requirement for arginine (ArgB-). A Chl- mutant gave rise spontaneously to ArgB- derivatives at high frequency. The same ArgB- requirement come out at high frequency among Chl- derivatives from a cross NFChl- x SCP1-Chl+ in which neither parent required arginine or produced spontaneously arginine-less derivatives. It is suggested that the Chl element is a "transposable element" (Tn) presumably associated with "insertion sequences" (IS). The insertional inactivation of the Chl element may be accompanied or followed by a deletion in the adjacent ArgB gene.