Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2

✍ Scribed by Usami, Yasuyuki ;Okamoto, Yoshiharu ;Takayama, Takahiro ;Shigemasa, Yoshihiro ;Minami, Saburo

• Book ID: 101258067
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 185 KB
• Volume: 42
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19981215)42:4<517::aid-jbm6>3.0.co;2-u

✦ Synopsis

The effects of chitin and chitosan on the release of arachidonic acid products were investigated in this study. Supernatants of canine polymorphonuclear cell (PMN) suspensions incubated with chitin and chitosan contained a leukotriene B 4 (LTB 4 ) concentration high enough to induce canine PMN migration in vitro. The supernatants also contained the same concentration of prostaglandin E 2 (PGE 2 ) as that normally found in the peripheral blood of dogs. Intraperitoneal administration of chitosan to dogs induced peri-toneal exudative fluid (PEF), but chitin did not. The PEF contained numerous PMNs and macrophages. The supernatant of PEF contained both heat-stable and heat-labile chemotactic factors for canine PMNs. It also contained enough LTB 4 to attract the canine PMNs in vitro.