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Chiral Recognition of O-Phosphoserine by Mass Spectrometry

✍ Scribed by Giovanna Fago; Antonello Filippi; Anna Giardini; Aldo Laganà; Alessandra Paladini; Maurizio Speranza

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
97 KB
Volume
40
Category
Article
ISSN
0044-8249

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✦ Synopsis

Identification and quantification of the enantiomeric excess of O-phosphoserine ( Ser) residues from post-translational modified proteins is now possible by the application of a very reproducible and sensitive mass spectrometric method. This technique is based on the different mass-spectral fragmentation patterns of the diastereomeric cluster ions generated in water/methanol solutions of the analyte ( Ser ) with a suitable chiral compound ( Thr ) by collision-induced dissociation (CID; see mass spectra).

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