Chiral gas chromatographic assay with flame ionization detection for amphetamine enantiomers in microsomal incubates

✍ Scribed by S. Zeng; L. Zhang; Y. Z. Chen

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 44 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199902)13:1<33::aid-bmc809>3.0.co;2-b

✦ Synopsis

A chiral assay for amphetamine enantiomers in rat liver microsomal incubates is based on derivatization with (S)-(À)-N-(trifluoroacetyl)-prolyl chloride (S-TFPC), capillary chromatographic separation of the diastereomeric amide derivatives, and detection by a flame ionization detector. The method is capable of detecting low levels of S-or R-amphetamine. The assay is linear from 5 to 250 mg/mL for each enantiomer, and the limit of detection is 0.5 mg/mL. The analytical method affords the average recoveries of 77.53 AE 5.22% for R-amphetamine and 74.47 AE 3.08% for S-amphetamine. The method allows the study of the metabolic depletion of S-and R-amphetamine in rat liver microsomal incubates. The time-dependent concentration of amphetamine enantiomers in rat liver microsomes was determined, and the stereoselectivity of amphetamine phase I metabolism was observed.