Chiral analysis of metoprolol and its by-products by capillary electrophoresis

✍ Scribed by Zoltán Juvancz; Karin E. Markides; László Jicsinszky

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 140 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:10<716::aid-mcs4>3.0.co;2-i

✦ Synopsis

The enantiomers of metoprolol, a frequently used ␤-blocker, have different physiological effects, which makes their chiral separation necessary. The separation of the main product from its by-products and their chiral separation Ž . Ž . were also aims of this study. Capillary electrophoresis CE with cyclodextrin CD additives, providing high efficiency and selectivity, was applied for this goal. Separation of the main product from 5 by-products as well as their chiral separa-Ž . tion was realized. Fifteen CDs native ␣, ␤, ␥ , and derivatives were evaluated. Ž . Ž . Carboxymethylated ␣-CD R 3.0 , and phosphated ␣-CD R 2.7 were the best s s

for the chiral separation of metoprolol while carboxymethylated ␣-CD, phosphated ␣-CD, and phosphated ␥-CD were appropriate for the enantioseparation of byproducts and for their separation from the main product. The roles of the type and concentration of the chiral selectors, of the pH, and of the organic additives in the buffers were studied. The stacking effect of phosphated ␣-CD was investigated in detail.