✦ LIBER ✦

Chemostat dynamics of plasmid-bearing, plasmid-free mixed recombinant cultures

✍ Scribed by Gregory Stephanopoulos; Gary R. Lapudis

Book ID: 103006533
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 876 KB
Volume: 43
Category: Article
ISSN: 0009-2509
DOI: 10.1016/0009-2509(88)87125-2

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✦ Synopsis

The problem of plasmid stability and strain reversion in recombinant cultures is investigated through a complete stability analysis of a plasmid-bearing, plasmid-free mixed culture growing in a chemostat.

Using a general method based on the index theory of a singular point, the complete stability portrait of all competitive interactions is obtained which can occur under all possible mutual dispositions of the specific growth rate curves and chemostat dilution rate. It is found that such a mixed culture can coexist in a chemostat only if there is a range of substrate concentrations where the plasmid-bearing cells grow at a specific rate which is larger than the specific growth rate of the plasmid-free cells. Realistic further genetic modifications that could possibly yield a culture with such properties are discussed.

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