ChemInform Abstract: α-Chymotrypsin-Catalyzed Peptide Synthesis Using Activated Esters as Acyl Donors.
✍ Scribed by T. MIYAZAWA; S. NAKAJO; M. NISHIKAWA; K. IMAGAWA; R. YANAGIHARA; T. YAMADA
- Publisher
- John Wiley and Sons
- Year
- 2010
- Weight
- 32 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0931-7597
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✦ Synopsis
Abstract
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The ability of the endopeptidase a-chymotrypsin (EC 3.4.21.1) to catalyse the reaction of various N aunprotected di-and tripeptide ester derivatives with H-Leu-NH 2 , and with a series of C-terminal free di-and tripeptides at ÿ15 C in frozen aqueous solution was investigated. The enzyme is able to s
Enzymatic Peptide Synthesis with p-Guanidinophenyl and p-(Guanidinomethyl)phenyl Esters as Acyl Donors. -Two series of "inverse substrates", e.g. (VII) and (IV), are prepared as acyl donor components for enzymatic peptide synthesis. These substrates are found to couple readily with amino acid p-nit