ChemInform Abstract: Synthesis of a Monocharged Peptide Nucleic Acid (PNA) Analogue and Its Recognition as Substrate by DNA Polymerases.

✍ Scribed by M. J. Lutz; D. W. Will; G. Breipohl; S. A. Brenner; E. Uhlmann

• Publisher: John Wiley and Sons
• Year: 2010
• Weight: 29 KB
• Volume: 30
• Category: Article
• ISSN: 0931-7597
• DOI: 10.1002/chin.199937242

✦ Synopsis

Synthesis of a Monocharged Peptide Nucleic Acid (PNA) Analogue and Its Recognition as Substrate by DNA Polymerases.

-The preparation of a novel phosphoramidite monomer based on thyminyl acetic acid coupled to a secondary nitrogen of 2-(2-amino-ethylamino)ethanol (III) is described. This monomer can be used to attach a deoxynucleotide to the carboxy terminus of a PNA oligomer by solid-phase synthesis. The resulting PNA primer is recognized as a substrate by various DNA polymerases. -(