ChemInform Abstract: Microbial Transformations: Production of D-Amino Acids Using Hydantoinase
β Scribed by Rakesh Sharma; Rakesh M. Vohra
- Publisher
- John Wiley and Sons
- Year
- 2010
- Weight
- 24 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0931-7597
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β¦ Synopsis
Abstract
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## Abstract It has been shown that resting cells of __Agrobacterium radiobacter__ catalyze a sequence of two stereospecific hydrolytic reactions leading to the complete transformation of racemic hydantoins to Dβamino acids. This report describes some properties of this new biocatalyst and its poten
A thermostable D-hydantoinase from Bacillus stearothermophilus SD-1 was previously mass-produced by batch cultivation of the recombinant E. coli harboring the gene encoding the enzyme (Lee et al., 1997). In this work, we attempted to optimize the process for the production of N-carbamoyl-D-p-hydroxy