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ChemInform Abstract: Further Studies on the Chemical Cleavage of an N-Terminal Extra Methionine from Recombinant Methionylated Proteins.

✍ Scribed by Masato Suenaga; Hiroaki Ohmae; Norio Okutani; Tomofumi Kurokawa; Tsuneo Asano; Takao Yamada; Osamu Nishimura; Masahiko Fujino

Publisher: John Wiley and Sons
Year: 2010
Weight: 29 KB
Volume: 31
Category: Article
ISSN: 0931-7597
DOI: 10.1002/chin.200017191

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Abstract

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An Efficient Chemical Method for Removing N-Terminal Extra Methionine from Recombinant Methionylated Human Growth Hormone. -The title process is successfully achieved via a transamination reaction and a phenylene-1,2-diamine treatment. The method may be of more general use for the preparation of no