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Chemiluminometric l-lysine determination with immobilized lysine oxidase by flow-injection analysis

✍ Scribed by F. Preuschoff; U. Spohn; E. Weber; K. Unverhau; K.-H. Mohr

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 407 KB
Volume: 280
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)85120-9

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✦ Synopsis

An enzymatic flow-injection procedure for the determination of L-lysine was developed. A lysine oxidase reactor is combined with a fibre-optic hydrogen peroxide detector. Hydrogen peroxide detection is based on the peroxidasecatalysed luminol reaction. The chemiluminescent light is detected by a photomultiplier. L-Lysine can be determined in the range 10-1000 PM. A sampling rate of up to 90 b-' can be achieved. The whole sensing assay works for more than 1 month. The double logarithmic graph of the peak signal height vs. the lysine concentration is linear, the slope being larger than unity (r* = 0.991, n = 4).

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