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Chemiluminescent assays for choline and phospholipase-D using a flow injection system

✍ Scribed by Yaqoob, M. ;Nabi, A. ;Masoom-Yasinzai, M.

Publisher: John Wiley and Sons
Year: 1997
Weight: 142 KB
Volume: 12
Category: Article
ISSN: 0884-3996
DOI: 10.1002/(sici)1099-1271(199705/06)12:3<135::aid-bio439>3.0.co;2-b

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✦ Synopsis

A flow injection chemiluminescent method is described for the determination of choline. The method is based on the production of hydrogen peroxide from choline using on-line covalently bound immobilized choline oxidase column. The product is mixed downstream and detected via the cobalt catalyzed chemiluminescent oxidation of luminol. The detection limit is 1 x 10(-7) mol/L, with rsd 1.8 to 2.8% in the range 2-10 x 10(-5) mol/L. The sample throughput is 30 per hour. The method was applied to the determination of choline produced off-line from phosphatidylcholine using phospholipase-D isolated from cabbage.

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