Chemiluminescence ELISA for the detection of antibodies to bovine leukaemia virus antigens

✍ Scribed by Vidžiūnaité, R. ;Dikiniené, N. ;Miliukiené, V. ;Mikulskis, P. ;Kulys, J.

• Publisher: John Wiley and Sons
• Year: 1995
• Weight: 424 KB
• Volume: 10
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170100308

✦ Synopsis

A chemiluminescence enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine leukaemia virus antigens (BLV) has been developed. The possibility o f using an enhanced chemiluminescence reaction for the determination of adsorbed immunoperoxidase conjugates was studied in this work.

The intensity o f chemiluminescence depends on both the concentration o f reagents and experimental conditions used. The efficiency o f the assay is determined by the formation o f an immobilized antigen monolayer. A relationship between the quant i t y o f the protein added and adsorbed has been shown. The optimal time and temperature for the antigen-antibody incubation steps have been estimated for each system ( 3 h a t 37°C was chosen as a standard incubation time). A linear dependence o f the chemiluminescence intensity and optical density on the concentration o f antibodies t o the BLV antigens was observed. The detection limit o f antibodies in the chemiluminescence ELISA is 2-3 times lower than t h a t in the spectrophotometric one. The results obtained indicate the possibility o f using both methods.