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Chemical modification and immobilisation of lipase B from Candida antarctica onto mesoporous silicates

✍ Scribed by Jessica Forde; Alex Vakurov; Tim D. Gibson; Paul Millner; Mícheál Whelehan; Ian W. Marison; Ciarán Ó’Fágáin

Book ID
113804686
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
249 KB
Volume
66
Category
Article
ISSN
1381-1177

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Alcohol inhibition and specificity studi
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## Abstract The engineering of lipase B from __Candida antarctica__ (CALB) by circular permutation has yielded over sixty hydrolase variants, and several show significantly improved catalytic performance. Here we report a detailed characterization of ten selected enzyme variants by kinetic and spec