Chemical and immunochemical investigation of the lipopolysaccharides ofVibrio alginolyticus
β Scribed by E. L. Nazarenko; R. P. Gorshkova; T. I. Burtseva; Yu. S. Ovodov
- Book ID
- 104828905
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 433 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0009-3130
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Smooth lipopolysaccharide (sLPS) of Brucella abortus was prepared and fractionated by a modification of the procedures of Moreno et al. (J. Bac. 138:361-369, 1979). Washed B. abortus cells were disrupted by 21 freeze-quick thaw cycles with ultrasonication to separate the non-membrane-bound material.
## Abstract Chemical analyses, NMR spectroscopy, and mass spectrometry were used to elucidate the structure of the rough lipopolysaccharide (LPS) isolated from __Acinetobacter lwoffii__β F78. As a prominent feature, the core region of this LPS contained the disaccharide Ξ±βKdoβ(2β8)βΞ±βKdo (Kdo=3βdeox