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Charomids: Cosmid Vectors for Efficient Cloning and Mapping of Large or Small Restriction Fragments

✍ Scribed by Izumu Saito and George R. Stark

Book ID: 123633998
Publisher: National Academy of Sciences
Year: 1986
Tongue: English
Weight: 1022 KB
Volume: 83
Category: Article
ISSN: 0027-8424
DOI: 10.2307/29115

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The production and use of the 6 kb cosmid pHC79, a derivative of pBR322, is described. It can be used for cloning of fragments cleaved by EcoRI, ClaI, BamHI (also BgllI, BclI, Sau3A and MboI), Sail (also XhoI and AvaI), EcaI and PstI. Hybrid cosmids containing inserts in the size range of 40 kb are