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A small cosmid for efficient cloning of large DNA fragments

โœ Scribed by Hohn, Barbara; Collins, John

Book ID
122742821
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
878 KB
Volume
11
Category
Article
ISSN
0378-1119

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A small cosmid for efficient cloning of
A small cosmid for efficient cloning of large DNA fragments
โœ Hohn, Barbara; Collins, John ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 878 KB

The production and use of the 6 kb cosmid pHC79, a derivative of pBR322, is described. It can be used for cloning of fragments cleaved by EcoRI, ClaI, BamHI (also BgllI, BclI, Sau3A and MboI), Sail (also XhoI and AvaI), EcaI and PstI. Hybrid cosmids containing inserts in the size range of 40 kb are