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Charge derivatization of peptides for analysis by mass spectrometry

✍ Scribed by Kenneth D. W. Roth; Zhi–Heng Huang; Nalini Sadagopan; J. Throck Watson

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
384 KB
Volume
17
Category
Article
ISSN
0277-7037

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✦ Synopsis

The analysis of peptide derivatives by fast atom bombardment, liquid secondary-ionization mass spectrometry, plasma desorption, electrospray ionization, and matrix-assisted laser desorption/ionization is reviewed. The fragmentation patterns of peptides and of charge-derivatized peptides are compared, and the proposed fragment ion structures are summarized. A variety of derivatization approaches and the distinguishing features of mass spectra produced from these derivatives are described. The most promising derivatization approaches are evaluated, and the strengths and limitations of these approaches are discussed.

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