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Characterization of the Protein Phosphatase 1-Binding Motifs of Inhibitor-2 and DARPP-32 by Surface Plasmon Resonance

✍ Scribed by Lin, T.-H.


Book ID
111882143
Publisher
Oxford University Press
Year
2005
Tongue
English
Weight
195 KB
Volume
138
Category
Article
ISSN
0021-924X

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Two biotin conjugates ofokadaic acid were synthesized for evaluating their interactions with protein phosphatase 2A by surface plasmon resonance (SPR). C7-biotinylated okadaic acid revealed strong binding affinity to the enzyme, while Cl-biotinylated derivative being devoid of affinity, implying tha