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Characterization of the molecular defect in a null allele of theopaque-2locus of maize

✍ Scribed by Gisela Hoschek; Kelly A. Russell; Robert J. Schmidt

Publisher: Springer
Year: 1996
Tongue: English
Weight: 319 KB
Volume: 32
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00041400

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✦ Synopsis

The molecular defect in an opaque-2 (02) mutant, previously characterized as a null allele, has been identified as containing an insertion of the transposable element of the Bergamo (Bg) family. Restriction mapping and partial sequence analysis of the Bg in the 02 null allele indicates that this element is distinct from the previously described Bg as well as the defective Bg (rbg) of the o2m(r) allele. It is, however, inserted at the same site in 02 as the rbg of o2m(r) and can transpose when Bg is present. This study shows that, depending on genetic background, this allele may not behave as a stable null which could dramatically influence the conclusions drawn from experiments based on this particular mutant.

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