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Characterization of the Hansenula polymorpha PUR7 gene and its use as selectable marker for targeted chromosomal integration

✍ Scribed by Gert Jan Haan; Ralf van Dijk; Jan A.K.W. Kiel; Marten Veenhuis


Book ID
109295034
Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
717 KB
Volume
2
Category
Article
ISSN
1567-1356

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A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic