Quantitation of FAD-Dependent Cytochrome
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Anne V. Hodgson; Henry W. Strobel
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Article
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1996
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Elsevier Science
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English
β 72 KB
NADPH cytochrome P450 reductase binds two flavin cofactors, FMN and FAD, per molecule of reductase. We have developed an assay to quantitate the reduction activity of FMN-bound flavoprotein. This Trislight assay system takes advantage of the ability of photoactivated flavins to release electrons to