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Characterization of the FAD Binding Domain of Cytochrome P450 Reductase

✍ Scribed by Anne V. Hodgson; Henry W. Strobel


Book ID
115565856
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
119 KB
Volume
325
Category
Article
ISSN
0003-9861

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Quantitation of FAD-Dependent Cytochrome
✍ Anne V. Hodgson; Henry W. Strobel πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 72 KB

NADPH cytochrome P450 reductase binds two flavin cofactors, FMN and FAD, per molecule of reductase. We have developed an assay to quantitate the reduction activity of FMN-bound flavoprotein. This Trislight assay system takes advantage of the ability of photoactivated flavins to release electrons to