Three new deletion mutants and an insertion mutant of E. coli bacteriophage P2, del2, vir79, del4 and sig5, were mapped by the electron microscope heteroduplex method. The deletions were found to cover 45.5-51.6%, 75.6-76.7% and 92.3-99.3% respectively of P2 DNA while sig5 represented a 3.7% inserti
Characterization of the DNA from bacteriophage P2-186 hybrids and physical mapping of the 186 chromosome
β Scribed by Younghusband, H. Banfield ;Egan, J. Barry ;Inman, Ross B.
- Publisher
- Springer
- Year
- 1975
- Tongue
- English
- Weight
- 814 KB
- Volume
- 140
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
The DNA from two P2-186 hybrid phages and three 186 Insertion mutants have been characterized by heteroduplex analysis and denaturation mapping. The results allow the orientation of the physical and genetic maps of bacteriophage 186 DNA and put physical limits on the chromosomal locations of the phage attachment sites, immunity genes and tail genees.
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