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Characterization of the Aspergillus nidulans biotin biosynthetic gene cluster and use of the bioDA gene as a new transformation marker

✍ Scribed by Pasqualina Magliano; Michel Flipphi; Dominique Sanglard; Yves Poirier


Book ID
116482118
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
1020 KB
Volume
48
Category
Article
ISSN
1087-1845

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A transformation system using resistance to the antibiotic cycloheximide as a dominant selectable marker was developed for the pathogenic yeast Cryptococcus neoformans. A 3.5 kb DNA fragment containing a gene encoding the ribosomal protein L41 was cloned from a wild-type strain of C. neoformans whic