The 12 S globulin of rapeseed represents an oligomeric protein with a molecular weight of 300,000. It is composed of 6 subunits, which are arranged in a trigonal. antiprism with the point group symmetry 32 (D3). Each subunit contains smaller units (polypeptide chains) with molecular weights in the
✦ LIBER ✦
Characterization of the 12S globulin complex of Brassica napus : Evolutionary relationship to other 11–12S storage globulins
✍ Scribed by Staffan SJÖDAHL; Joakim RÖDIN; Lars RASK
- Book ID
- 115128478
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 612 KB
- Volume
- 196
- Category
- Article
- ISSN
- 1432-1327
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## Abstract An isolation procedure for the 12 S rapeseed globulin is described which includes precipitation by dialysis, purification using gel chromatography on Sephadex G‐200, and ion‐exchange chromatography on DEAE‐Sephadex A‐50. The isolated globulin represents a neutral protein with an isoele