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Characterization of the 12S globulin complex of Brassica napus : Evolutionary relationship to other 11–12S storage globulins

✍ Scribed by Staffan SJÖDAHL; Joakim RÖDIN; Lars RASK


Book ID
115128478
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
612 KB
Volume
196
Category
Article
ISSN
1432-1327

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The structure of the 12 S globulin from
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The 12 S globulin of rapeseed represents an oligomeric protein with a molecular weight of 300,000. It is composed of 6 subunits, which are arranged in a trigonal. antiprism with the point group symmetry 32 (D3). Each subunit contains smaller units (polypeptide chains) with molecular weights in the

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## Abstract An isolation procedure for the 12 S rapeseed globulin is described which includes precipitation by dialysis, purification using gel chromatography on Sephadex G‐200, and ion‐exchange chromatography on DEAE‐Sephadex A‐50. The isolated globulin represents a neutral protein with an isoele