Characterization of Self-Assembling of Glucose Dehydrogenase in Mono- and Multilayers on Gold Electrodes

✍ Scribed by Noya Loew; Frieder W. Scheller; Ulla Wollenberger

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 115 KB
• Volume: 16
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200403005

✦ Synopsis

Abstract

Glucose dehydrogenase (GDH) was assembled electrostatically onto QCM‐gold electrodes by their sequential deposition with anionic polyelectrolytes such as PSS and PASA. For the layer‐by‐layer arrangements both the microgravimetric and the electrochemical sensor signal were followed. Increasing amounts of GDH were deposited by stepwise formation of alternating layers of GDH and PSS or PASA. The mass increase was about 1.88 μg/cm^2^ for one GDH/PASA bilayer and 2.4 μg/cm^2^ for a GDH/PSS bilayer. The addition of phenolic compounds resulted in an oxidation current, which could be catalytically increased by the GDH catalysed reaction in the presence of glucose. The system functions as glucose sensor when quinones are present in nonlimiting amount. The amperometric response was already diffusion limited when a single layer of GDH was adsorbed. The sensor sensitivity increased by a factor of 10 when MSA was used instead of MUA as initial electrode modifier.