✦ LIBER ✦

Characterization of Saccharomyces Sp. through determination of amino acid profiles by capillary gas chromatography

✍ Scribed by Vasconcelos, A. M. P. ;Neves, H. J. Chaves Das

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 309 KB
Volume: 8
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240080914

No coin nor oath required. For personal study only.

✦ Synopsis

The Saccharomyces strains uvarum, montuliensis, bayanus, capensis and florentinus were grown in liquid media prepared from a 1% aqueous glucose solution to which 0.05% of ammonium sulfate or urea were added as the only nitrogen supply. The amino acids exuded during incubation were isolated by cation exchange and derivatized as the corresponding N,O-heptafluorobutyryl isopropyl esters. Separation was achieved on a highly deactivated glass capillary column coated with OV-101. A characteristic amino acid profile was obtained for each of the species studied, allowing the construction of a chemotaxonomic tree, on the basis of present or absent acids.

📜 SIMILAR VOLUMES

Determination of polyol profiles in huma

Determination of polyol profiles in human urine by capillary gas chromatography

✍ Hidehiko Haga; Terumi Nakajima 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 344 KB 👁 2 views

Determination of amino acids by gas chro

Determination of amino acids by gas chromatography using a wide bore capillary column

✍ Moodie, I. M. ;Burger, J. A. ;Labadarios, D. 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 429 KB 👁 2 views

A procedure is described in whicha wide borecapillarycolumn is used as an alternative to the more traditional packed column for the quantitative analysis of amino acids as their N-heptafluorobutyryl isobutyl ester (HBB) derivatives. Thecolumn, installed in a gas chromatograph previously configured f

Determination of amino acids in urine by

Determination of amino acids in urine by gas chromatography

✍ Moodie, I. M. ;Hough, B. J. ;Labadarios, D. 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 425 KB 👁 1 views

This report describes a convenient means of reducing the complex matrix which is responsible for interference during gas chromatographic determination of amino acids in urine.The pre-chromatographic clean-up employs, the principle of solid phase extraction using bonded silica incorporating cation ex

Determination of ethylenediaminetetraace

Determination of ethylenediaminetetraacetic acid and related amino-carboxylic acids by gas chromatography

✍ Subach, D. J. ;James, J. E. 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 149 KB 👁 1 views

Determination of amino acids in human ce

Determination of amino acids in human cerebrospinal fluid by gas chromatography

✍ Labadarios, D. ;Moodie, I. M. ;Hough, B. J. ;Burger, J. A. 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 384 KB

In the present study, the use of gas chromatography (GC) for the determination of amino acids in human cerebrospinal fluid (CSF) is described. Although some amino acids may be determined using a packed column following the removal of glucose, the major interfering component, the inadequate resolutio

Gas chromatography of amino acids: Use o

Gas chromatography of amino acids: Use of a fused-silica capillary column in the determination of plasma amino acids

✍ Labadarios, D. ;Moodie, I. M. ;Burger, J. ;Shephard, G. S. 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 411 KB 👁 1 views

A capillary chromatographic procedure using a fused silica column is described which can be used to quantitatively determine amino acids in plasma following the pre-chromatographic "clean-up" described in a recent paper [l]. In substituting this procedure for that involving a packed column, advantag