Rapid purification and partial character
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V. B. Yadwad; K. Meghji; O. P. Ward
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Article
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1993
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Springer-Verlag
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English
โ 542 KB
Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands