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Characterization of recombinant human epidermal growth factor produced in yeast

โœ Scribed by George-Nascimento, Carlos; Gyenes, Alexander; Halloran, S. Mitchell; Merryweather, James; Valenzuela, Pablo; Steimer, Kathelyn S.; Masiarz, Frank R.; Randolph, Anne


Book ID
126265257
Publisher
American Chemical Society
Year
1988
Tongue
English
Weight
726 KB
Volume
27
Category
Article
ISSN
0006-2960

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Rapid purification and partial character
โœ V. B. Yadwad; K. Meghji; O. P. Ward ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 542 KB

Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands