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Characterization of recombinant fructose-1,6-bisphosphate aldolase fromMethylococcus capsulatusBath

✍ Scribed by O. N. Rozova; V. N. Khmelenina; I. I. Mustakhimov; A. S. Reshetnikov; Y. A. Trotsenko


Book ID
110155412
Publisher
Springer
Year
2010
Tongue
English
Weight
288 KB
Volume
75
Category
Article
ISSN
0006-2979

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A rapid procedure for the purification of fructose-l,6-bisphosphate aldolase from spinach chloroplasts is presented which involves two steps; precipitation of bulk protein with polyethylene glycol and partitioning of remaining soluble protein in aqueous two-phase systems. A 94% pure preparation is o