Characterization of protein kinase C activity in interferon gamma treated murine peritoneal macrophages
β Scribed by David L. Becton; Dolph O. Adams; Thomas A. Hamilton
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 745 KB
- Volume
- 125
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Treatment of M5076 tumor cells with the phorbol esters 12-0-tetradecanoylphorbol 13-acetate (TPA) and phorbol 12,13 dibutyrate (PdBu) inhibited cellular proliferation, whereas 1,2-dioctanoyl-glycerol (DiC8) and 1 -0leoyl2-acetyl-glycerol (OAG) did not affect cell growth. Inhibition uf cellular proli
## Abstract Cyclic adenosine 3β²:5β²βmonophosphate (cAMP)βdependent protein kinases from three clones of C1300 neuroblastoma, established as growing tumors in A/jax mice, were identified and characterized. Mean (Β± SD) intratumor concentrations of cAMP ranged from 5.0Β±2.4 to 6.8Β±1.9 pmol/mg protein; c
DEAE-cellulose chromatography of the 20,000g supernatant fraction of homogenates of C-1300 murine neuroblastoma (clone N2a) yields one major and two minor peaks of cyclic AMP-dependent protein kinase activity. Assessment of the endogenous activation state of the enzyme(s) reveals that the enzyme is