Characterization of Polyphenol Oxidase from the Latex of Opium Poppy
✍ Scribed by F. Bilka; A. Balažová; A. Bilková; Z. Šubr; M. Pšenák
- Book ID
- 111568812
- Publisher
- Springer Netherlands
- Year
- 2004
- Tongue
- English
- Weight
- 619 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0006-3134
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Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6 ~o nucleotide and 84.6 ~o amino acid sequence identity with previously isolated clones. DNA
## Abstract Polyphenol oxidase (PPO), the enzyme responsible for the postharvest spoilage of fruits, was extracted and purified from __Uapaca kirkiana__ peel and pulp by ammonium sulfate precipitation and dialysis. Further purification of peel PPO was carried out by gel filtration chromatography. O